Molecular contrast on phase-contrast microscope

TL;DR

This paper introduces a simple, attachable molecular-contrast unit for standard phase-contrast microscopes, enabling high-speed, label-free molecular imaging of biological cells without damaging samples or requiring expensive equipment.

Contribution

The authors develop and demonstrate a low-cost, easy-to-integrate molecular-contrast module that enhances existing microscopes with molecular imaging capabilities.

Findings

Achieved high-speed, label-free molecular contrast imaging.

Demonstrated compatibility with standard phase-contrast microscopes.

Enabled molecular imaging with low-power optical illumination.

Abstract

An optical microscope enables image-based findings and diagnosis on microscopic targets, which is indispensable in many scientific, industrial and medical settings. Majority of microscope users are accustomed to standard benchtop microscopes, but it fails to give molecular contrast of the specimen which otherwise requires expensive specialized instruments to measure, accompanied by chemical or optical damages to the sample and/or slow imaging speed. Here, we report on a simple optical instrument, comprising of a semiconductor amplitude-modulated mid-infrared quantum cascade laser, that is attached to a standard microscope to deliver the additional molecular contrast of the specimen on top of its conventional microscopic image. We attach this instrument, termed molecular-contrast unit, to a standard phase-contrast microscope, and demonstrate high-speed label-free molecular-contrast phase-contrast imaging of biological cells under low-power optical illumination. Our simple molecular-contrast unit can empower existing standard microscopes of various users, by delivering a convenient accessibility to the molecular world.