Highly stable common-path quantitative phase microscope for biomedical imaging

TL;DR

This paper presents a highly stable, cost-effective common-path quantitative phase microscope capable of measuring membrane fluctuations in biological cells with high temporal stability, suitable for biomedical diagnostics.

Contribution

The authors developed a single-element, highly stable common-path QPM system that achieves ~15 mrad stability without vibration isolation, enabling accurate biological measurements.

Findings

Achieved ~15 mrad temporal stability without vibration isolation.

Successfully measured membrane fluctuations in human RBCs (~63 nm).

Demonstrated system's effectiveness on resolution charts and biological samples.

Abstract

High temporal stability is the primary requirement of any quantitative phase microscope (QPM) systems for the early stage detection of various human related diseases. The high temporal stability of the system provides accurate measurement of membrane fluctuations of the biological cells, which can be good indicator of various diseases. We developed a single element highly stable common-path QPM system to obtain temporally stable holograms of the biological specimens. With the proposed system, the temporal stability is obtained ~ 15 mrad without using any vibration isolation table. The capability of the proposed system is demonstrated on USAF resolution chart, polystyrene spheres (dia. 4.5 micron) and human red blood cells (RBCs). The membrane fluctuation of healthy human RBCs is further successfully measured and found to be equal to 63 nm. Contrary to its counterparts, present system offers energy efficient, cost effective and simple way of generating object and reference beam for the development of common-path QPM.