Adapting a plant tissue model to animal development: introducing cell sliding into VirtualLeaf

TL;DR

This paper extends the VirtualLeaf model, originally for plant tissues, by introducing cell sliding rules to better simulate animal tissue development, enabling more realistic modeling of cell rearrangements and mechanics.

Contribution

It introduces a novel cell sliding rule into VirtualLeaf, allowing the model to simulate cell rearrangements like T1 and T2 transitions in animal tissues.

Findings

VirtualLeaf produces different results in cell sorting compared to traditional models.

The new model captures cell boundary buckling and sliding behaviors.

It enables simulation of tissue mechanics under compression.

Abstract

Cell-based, mathematical modeling of collective cell behavior has become a prominent tool in developmental biology. Cell-based models represent individual cells as single particles or as sets of interconnected particles, and predict the collective cell behavior that follows from a set of interaction rules. In particular, vertex-based models are a popular tool for studying the mechanics of confluent, epithelial cell layers. They represent the junctions between three (or sometimes more) cells in confluent tissues as point particles, connected using structural elements that represent the cell boundaries. A disadvantage of these models is that cell-cell interfaces are represented as straight lines. This is a suitable simplification for epithelial tissues, where the interfaces are typically under tension, but this simplification may not be appropriate for mesenchymal tissues or tissues that are under compression, such that the cell-cell boundaries can buckle. In this paper we introduce a variant of VMs in which this and two other limitations of VMs have been resolved. The new model can also be seen as on off-the-lattice generalization of the Cellular Potts Model. It is an extension of the open-source package VirtualLeaf, which was initially developed to simulate plant tissue morphogenesis where cells do not move relative to one another. The present extension of VirtualLeaf introduces a new rule for cell-cell shear or sliding, from which T1 and T2 transitions emerge naturally, allowing application of VirtualLeaf to problems of animal development. We show that the updated VirtualLeaf yields different results than the traditional vertex-based models for differential-adhesion-driven cell sorting and for the neighborhood topology of soft cellular networks.