Development of Carbon-Fiber Electrodes Modified for Electrochemical ATP Detection

TL;DR

This paper reports the development of modified carbon-fiber microelectrodes for local, electrochemical detection of ATP, enabling more precise measurement of this vital molecule in biological systems.

Contribution

The study introduces a novel electrode modification technique that allows for direct electrochemical detection of ATP at specific locations.

Findings

Successful fabrication of carbon-fiber microelectrodes

Surface modification enables ATP detection

Potential for real-time, localized ATP measurement

Abstract

Adenosine triphosphate (ATP) is a highly energetic molecule used as a coenzyme in the transfer of chemical energy required for the continuation of cellular metabolism. In addition, extracellular ATP has an important role in cell-to-cell communication in immune, nervous and vascular systems. ATP is detected mainly using commercially available luciferin-luciferase kits and this method does not allow local measurement. In this study, first carbon-fiber microelectrodes capable of local detection were fabricated and then, the surface of these electrodes were modified with enzymes for detection of non-electroactive ATP molecules.