Multi-purpose SLM-light-sheet microscope

TL;DR

This paper presents a versatile SLM-integrated light-sheet microscope capable of multiple advanced imaging techniques, improving image quality and flexibility while maintaining simplicity and compatibility with open-source designs.

Contribution

The authors developed an easy-to-build, multi-modal light-sheet microscope using a phase-only SLM, enabling various imaging techniques on a single, open-source compatible setup.

Findings

Demonstrated high-quality imaging on diverse biological samples.

Showed improved image quality through techniques like structured illumination and pivoting.

Achieved flexible, multi-modal imaging with a simple, adaptable system.

Abstract

By integrating a phase-only Spatial Light Modulator (SLM) into the illumination arm of a cylindrical-lens-based Selective Plane Illumination Microscope (SPIM), we have created a versatile system able to deliver high quality images by operating in a wide variety of different imaging modalities. When placed in a Fourier plane, the SLM permits modulation of the microscope's light-sheet to implement imaging techniques such as structured illumination, tiling, pivoting, autofocusing and pencil beam scanning. Previous publications on dedicated microscope setups have shown how these techniques can deliver improved image quality by rejecting out-offocus light (structured illumination and pencil beam scanning), reducing shadowing (light-sheet pivoting), and obtaining a more uniform illumination by moving the highest-resolution region of the light-sheet across the imaging Field of View (tiling). Our SLM-SPIM configuration is easy to build and use, and has been designed to allow all of these techniques to be employed on one optical setup compatible with the OpenSPIM design. It also offers the possibility to choose between three different light-sheets, in thickness and height, which can be selected according to the characteristics of the sample and the imaging technique to be applied. We demonstrate the flexibility and performance of the system with results obtained by applying a variety of different imaging techniques on samples of fluorescent beads, Zebrafish embryos, and optically cleared whole mouse brain samples. Thus our approach allows easy implementation of advanced imaging techniques while retaining the simplicity of a cylindrical-lens-based light-sheet microscope.