Methods of electron microscopy of biological and abiogenic structures in artificial gas atmospheres

TL;DR

This paper reviews electron microscopy techniques in artificial gas atmospheres, focusing on biological and abiogenic structures, and discusses how varying environmental conditions affect analysis and modifications.

Contribution

It introduces methods for electron microscopy in controlled gas atmospheres, highlighting approaches for manipulating biological and abiogenic structures under different environmental parameters.

Findings

Varying gas, temperature, humidity, and pressure affects biological structure analysis.

Kinetic and dynamic approaches enable tissue and bioinorganic structure manipulation.

Simulation of beam-induced formation and disintegration of abiogenetic structures is feasible.

Abstract

This paper reviews opportunities for using electron microscopy in various gas atmospheres for the analysis and morpho-physiological modification of biological structures. The approaches that allow varying the gaseous phase content, as well as temperature, humidity, and pressure, are considered. The applicability of both kinetic and dynamic approaches to the tissue and bioinorganic structure manipulations is pointed out. The possibility of simulation of the beam-induced formation and disintegration of abiogenetic molecular structures is also mentioned as a particular case of the electron beam influence and treatment of the precursor medium in an artificial atmosphere.