Low-coherent optical diffraction tomography by angle-scanning illumination

TL;DR

This paper introduces a novel low-coherent optical diffraction tomography method using angle-scanning interferometry, enabling detailed 3D imaging of biological samples with incoherent light.

Contribution

It presents a new experimental setup and reconstruction algorithms for low-coherent ODT, expanding the capabilities of optical imaging with incoherent illumination.

Findings

Successful imaging of microspheres, red blood cells, and eukaryotic cells.

Maintains full-field interference during scans with incoherent light.

Addresses limitations of incoherent illumination in ODT.

Abstract

We propose and experimentally demonstrate temporally low-coherent optical diffraction tomography (ODT) based on angle-scanning Mach-Zehnder interferometry. Using a digital micromirror device based on diffractive tilting, we successfully maintain full-field interference of incoherent light during every scan sequence. The ODT reconstruction principles for temporally incoherent illuminations are thoroughly reviewed and developed. Several limitations of incoherent illumination are also discussed, such as the nondispersive assumption, optical sectioning capacity, and illumination angle limitation. Using the proposed setup and reconstruction algorithms, we successfully demonstrate low-coherent ODT imaging of microspheres, human red blood cells, and eukaryotic cells.