Microfluidic study of effects of flow velocity and nutrient concentration on biofilm accumulation and adhesive strength in a microchannel

TL;DR

This study investigates how flow velocity and nutrient concentration affect biofilm growth and adhesion in microchannels, providing insights for bioplugging applications in porous media.

Contribution

It offers experimental insights into biofilm behavior under different hydrodynamic conditions, aiding in modeling bioplugging in porous systems.

Findings

Higher flow velocity promotes biofilm growth up to a threshold.

Excessive shear stress causes biofilm detachment.

High nutrient levels lead to weaker biofilm adhesion.

Abstract

Biofilm accumulation in the porous media can cause plugging and change many physical properties of porous media. Targeted bioplugging may have significant applications for industrial processes. A deeper understanding of the relative influences of hydrodynamic conditions including flow velocity and nutrient concentration, on biofilm growth and detachment is necessary to plan and analyze bioplugging experiments and field trials. The experimental results by means of microscopic imaging over a T-shape microchannel show that increase in fluid velocity could facilitate biofilm growth, but that above a velocity threshold, biofilm detachment and inhibition of biofilm formation due to high shear stress were observed. High nutrient concentration prompts the biofilm growth, but was accompanied by a relatively weak adhesive strength. This letter provides an overview of biofilm development in a hydrodynamic environment for better predicting and modelling the bioplugging associated with porous system in petroleum industry, hydrogeology, and water purification.