ssDNA sequencing by rectification

TL;DR

This paper introduces a nanopore sequencing method using side-embedded N-terminated carbon nanotube electrodes, leveraging rectification ratios for high specificity and resolution in DNA base identification.

Contribution

It presents a novel sequencing protocol employing rectification effects in nanotube electrodes, enhancing sensitivity, selectivity, and robustness over existing methods.

Findings

Rectification ratio correlates with nucleobase identity.

The method achieves high resolution and selectivity.

Robustness to molecular orientation is demonstrated.

Abstract

Fast, reliable and inexpensive DNA sequencing is an important pursuit in healthcare, especially in personalized medicine with possible deep societal impacts. Despite significant progress of various nanopore-based sequencing configurations, challenges remain in resolution (due to thermal fluctuations or to sensitivity to molecular orientation) and speed, which are calling for new approaches. Here we propose a sequencing protocol for DNA translocation through a nanopore with side-embedded N-terminated carbon nanotube electrodes. Employing DFT and Non-Equilibrium Green's Function formalism, we show that the rectification ratio (response to square pulses of alternating bias) bears high nucleobase specificity. The rectification arises due to bias-dependent resistance asymmetry on the deoxyribonucleotide-electrode interfaces. The asymmetry induces molecular charging and HOMO pinning to the electrochemical potential of one of the electrodes, assisted by an in-gap electric field effect caused by dipoles at the terminated electrode ends. This sequencing protocol is sensitive, selective with orders of magnitude, has high resolution, and it is robust to molecular orientation.