A UV-cured nanofibrous membrane of vinylbenzylated gelatin-poly({\epsilon}-caprolactone) dimethacrylate co-network by scalable free surface electrospinning

TL;DR

This study presents a scalable method to produce UV-cured, water-stable nanofibrous membranes from functionalized gelatin and PCL, enhancing their mechanical and thermal properties for biomedical use.

Contribution

It introduces a novel UV-curing process for gelatin-based nanofibers with covalent integration of PCL, improving stability and properties for regenerative applications.

Findings

Membranes did not dissolve in water after UV curing.

Enhanced thermal and mechanical properties observed.

Membranes supported cell viability in vitro.

Abstract

Electrospun nanofibrous membranes of natural polymers, such as gelatin, are fundamental in the design of regenerative devices. Crosslinking of electrospun fibres from gelatin is required to prevent dissolution in water, to retain the original nanofibre morphology after immersion in water, and to improve the thermal and mechanical properties, although this is still challenging to accomplish in a controlled fashion. In this study, we have investigated the scalable manufacture and structural stability in aqueous environment of a UV-cured nanofibrous membrane fabricated by free surface electrospinning (FSES) of aqueous solutions containing vinylbenzylated gelatin and poly(epsilon-caprolactone) dimethacrylate (PCL-DMA). Vinylbenzylated gelatin was obtained via chemical functionalisation with photopolymerisable 4-vinylbenzyl chloride (4VBC) groups, so that the gelatin and PCL phase in electrospun fibres were integrated in a covalent UV-cured co-network at the molecular scale, rather than being simply physically mixed. UV-cured nanofibrous membranes did not dissolve in water and showed enhanced thermal and mechanical properties, with respect to as-spun samples, indicating the effectiveness of the photo-crosslinking reaction. In addition, UV-cured gelatin/PCL membranes displayed increased structural stability in water with respect to PCL-free samples and were highly tolerated by G292 osteosarcoma cells. These results therefore support the use of PCL DMA as hydrophobic, biodegradable crosslinker and provide new insight on the scalable design of water insoluble, mechanical competent gelatin membranes for healthcare applications.