Cell Identity Codes: Understanding Cell Identity from Gene Expression Profiles using Deep Neural Networks

TL;DR

This paper introduces a deep learning approach using autoencoders to encode gene expression profiles into a compact cell identity code, enabling accurate cell type classification and biological interpretation.

Contribution

The study develops a novel deep autoencoder architecture that encodes gene expression profiles into a 30-dimensional cell identity code, improving cell type discrimination and biological understanding.

Findings

CIC can accurately reproduce original GEPs.

CIC encodes biological pathways and processes.

Model generalizes to unseen cell types.

Abstract

Understanding cell identity is an important task in many biomedical areas. Expression patterns of specific marker genes have been used to characterize some limited cell types, but exclusive markers are not available for many cell types. A second approach is to use machine learning to discriminate cell types based on the whole gene expression profiles (GEPs). The accuracies of simple classification algorithms such as linear discriminators or support vector machines are limited due to the complexity of biological systems. We used deep neural networks to analyze 1040 GEPs from 16 different human tissues and cell types. After comparing different architectures, we identified a specific structure of deep autoencoders that can encode a GEP into a vector of 30 numeric values, which we call the cell identity code (CIC). The original GEP can be reproduced from the CIC with an accuracy comparable to technical replicates of the same experiment. Although we use an unsupervised approach to train the autoencoder, we show different values of the CIC are connected to different biological aspects of the cell, such as different pathways or biological processes. This network can use CIC to reproduce the GEP of the cell types it has never seen during the training. It also can resist some noise in the measurement of the GEP. Furthermore, we introduce classifier autoencoder, an architecture that can accurately identify cell type based on the GEP or the CIC.