An Analysis Scheme for Investigation of Effects of Various Parameters on Signals in Acoustic-Resolution Photoacoustic Microscopy of Mice Brain: a Simulation Study

TL;DR

This paper proposes a hybrid analysis scheme combining time and frequency domains to study how parameters like depth, focal size, and contrast agent concentration affect signals in photoacoustic microscopy of mice brains.

Contribution

It introduces a novel hybrid analysis method that enhances understanding of parameter effects on photoacoustic signals in microscopy.

Findings

The scheme effectively differentiates effects of various parameters.

It provides detailed insights into signal variations with parameter changes.

The method improves analysis beyond simple frequency component observation.

Abstract

Photoacoustic spectral analysis is a novel tool for studying various parameters affecting signals in Photoacoustic microscopy. But only observing frequency components of photoacoustic signals doesn't make enough data for a desirable analysis. Thus a hybrid time-domain and frequency-domain analysis scheme has been proposed to investigate effects of various parameters like depth of microscopy, laser focal spot size and contrast agent concentration on Photoacoustic signals.