Membrane Trafficking in the Yeast Saccharomyces cerevisiae Model

TL;DR

This paper reviews the well-characterized membrane trafficking pathways in yeast Saccharomyces cerevisiae, highlighting its role as a model for understanding eukaryotic secretory and endocytic processes, and the key effectors involved.

Contribution

It provides a comprehensive overview of yeast membrane trafficking pathways, emphasizing their conservation and significance in eukaryotic cell biology.

Findings

Identification of key effectors and pathways in yeast trafficking

Conservation of trafficking mechanisms across eukaryotes

Historical context of secretory pathway discovery

Abstract

The yeast Saccharomyces cerevisiae is one of the best characterized eukaryotic models. The secretory pathway was the first trafficking pathway clearly understood mainly thanks to the work done in the laboratory of Randy Schekman in the 1980s. They have isolated yeast sec mutants unable to secrete an extracellular enzyme and these SEC genes were identified as encoding key effectors of the secretory machinery. For this work, the 2013 Nobel Prize in Physiology and Medicine has been awarded to Randy Schekman; the prize is shared with James Rothman and Thomas S{\"u}dhof. Here, we present the different trafficking pathways of yeast S. cerevisiae. At the Golgi apparatus newly synthesized proteins are sorted between those transported to the plasma membrane (PM), or the external medium, via the exocytosis or secretory pathway (SEC), and those targeted to the vacuole either through endosomes (vacuolar protein sorting or VPS pathway) or directly (alkaline phosphatase or ALP pathway). Plasma membrane proteins can be internalized by endocytosis (END) and transported to endosomes where they are sorted between those targeted for vacuolar degradation and those redirected to the Golgi (recycling or RCY pathway). Studies in yeast S. cerevisiae allowed the identification of most of the known effectors, protein complexes, and trafficking pathways in eukaryotic cells, and most of them are conserved among eukaryotes.