Spatial heterogeneities shape collective behavior of signaling amoeboid cells

TL;DR

This study investigates how spatial heterogeneities like pillars influence the pattern formation and collective signaling behavior of Dictyostelium amoebae, combining experiments and simulations to reveal the role of obstacles in wave dynamics.

Contribution

It provides novel experimental and computational insights into how physical obstacles affect cAMP wave initiation and propagation in signaling amoebae.

Findings

Pillars act as wave sources under certain boundary conditions.

Caffeine reduces the number of firing centers and alters wave dynamics.

Obstacles require a critical cAMP accumulation to generate waves.

Abstract

We present novel experimental results on pattern formation of signaling Dictyostelium discoideum amoeba in the presence of a periodic array of millimeter-sized pillars. We observe concentric cAMP waves that initiate almost synchronously at the pillars and propagate outwards. These waves have higher frequency than the other firing centers and dominate the system dynamics. The cells respond chemotactically to these circular waves and stream towards the pillars, forming periodic Voronoi domains that reflect the periodicity of the underlying lattice. We performed comprehensive numerical simulations of a reaction-diffusion model to study the characteristics of the boundary conditions given by the obstacles. Our simulations show that, the obstacles can act as the wave source depending on the imposed boundary condition. Interestingly, a critical minimum accumulation of cAMP around the obstacles is needed for the pillars to act as the wave source. This critical value is lower at smaller production rates of the intracellular cAMP which can be controlled in our experiments using caffeine. Experiments and simulations also show that in the presence of caffeine the number of firing centers is reduced which is crucial in our system for circular waves emitted from the pillars to successfully take over the dynamics. These results are crucial to understand the signaling mechanism of Dictyostelium cells that experience spatial heterogeneities in its natural habitat.