Microliter viscometry using a bright-field microscope: $\eta$-DDM

TL;DR

This paper introduces a novel microliter viscometry method, $ ext{η}$-DDM, using bright-field microscopy and Differential Dynamic Microscopy to measure rheological properties with minimal sample volume, applicable to biological and soft matter systems.

Contribution

The paper presents $ ext{η}$-DDM, a new microliter viscometry technique combining bright-field microscopy and DDM for reliable rheological measurements with very small samples.

Findings

$ ext{η}$-DDM accurately measures viscosity in various fluids.

The method requires only about one microliter of sample.

It is suitable for biological and soft matter applications.

Abstract

Passive microrheology exploits the Brownian motion of colloidal tracer particles. From the mean-squared displacement (MSD) of the tracers, the bulk rheological and viscometric properties of the host medium can be inferred. Here, the MSD is determined by applying Differential Dynamic Microscopy (DDM). Compared to other microscopy techniques, DDM avoids particle tracking but provides parameters commonly acquired in light scattering experiments. Based on the spatial Fourier transform of image differences, the intermediate scattering function and subsequently the MSD is calculated. Then the usual microrheology procedure and the empirical Cox-Merz rule yield the steady-shear viscosity. This method, $\eta$-DDM, is tested and illustrated using three different systems: Newtonian fluids (glycerol-water mixtures), colloidal suspensions (protein samples) and a viscoelastic polymer solution (aqueous poly(ethylene oxide) solution). These tests show that common lab equipment, namely a bright-field optical microscope, can be used as a convenient and reliable microliter viscometer. Because $\eta$-DDM requires much smaller sample volumes than classical rheometry, only about a microliter, it is particularly useful for biological and soft matter systems.