Water Bridging Dynamics of Polymerase Chain Reaction in the Gauge Theory Paradigm of Quantum Fields

TL;DR

This paper explores how water mediates DNA-enzyme interactions in PCR through electromagnetic fields, proposing a gauge theory framework to explain the high efficiency and specificity of Taq polymerase activity.

Contribution

It introduces a novel gauge theory paradigm to describe water's role in DNA-enzyme interactions and demonstrates experimental PCR amplification using electromagnetic signals from DNA.

Findings

Water mediates DNA-enzyme interactions via electromagnetic fields.

PCR can be achieved with electromagnetic signals from DNA in water.

The gauge theory framework explains PCR efficiency and specificity.

Abstract

We discuss the role of water bridging the DNA-enzyme interaction by resorting to recent results showing that London dispersion forces between delocalized electrons of base pairs of DNA are responsible for the formation of dipole modes that can be recognized by \textit{Taq} polymerase. We describe the dynamic origin of the high efficiency and precise targeting of \textit{Taq} activity in PCR. The spatiotemporal distribution of interaction couplings, frequencies, amplitudes, and phase modulations comprise a pattern of fields which constitutes the electromagnetic image of DNA in the surrounding water, which is what the polymerase enzyme actually recognizes in the DNA water environment. The experimental realization of PCR amplification, achieved through replacement of the DNA template by the treatment of pure water with electromagnetic signals recorded from viral and bacterial DNA solutions, is found consistent with the gauge theory paradigm of quantum fields.