Inferring neuronal couplings from spiking data using a systematic procedure with a statistical criterion

TL;DR

This paper introduces a systematic, statistically rigorous method for inferring neuronal couplings from spike train data, effectively reducing false positives and accurately identifying synaptic connections.

Contribution

It proposes a novel two-step procedure for data preprocessing and coupling screening, improving inference accuracy in neuronal network analysis.

Findings

Accurately identifies presence and sign of synaptic couplings

Reduces false positives through randomized data comparison

Effective on both synthetic and real neuronal data

Abstract

Recent remarkable advances in the experimental techniques have provided a background for inferring neuronal couplings from point process data that includes a great number of neurons. Here, we propose a systematic procedure for pre- and post-processing generic point process data in an objective manner, to handle data in the framework of a binary simple statistical model, the Ising or generalized McCulloch--Pitts model. The procedure involves two steps: (1) determining time-bin size for transforming the point-process data into discrete-time binary data and (2) screening relevant couplings from the estimated couplings. For the first step, we decide the optimal time-bin size by introducing the null hypothesis that all neurons would fire independently, then choosing a time-bin size so that the null hypothesis is rejected with the most strict criterion. The likelihood associated with the null hypothesis is analytically evaluated and used for the rejection process. For the second post-processing step, after a certain estimator of coupling is obtained based on the pre-processed dataset, the estimate is compared with many other estimates derived from datasets obtained by randomizing the original dataset in the time direction. We accept the original estimate as relevant only if its absolute value is sufficiently larger than them of randomized datasets. These manipulations suppress false positive couplings induced by statistical noise. We apply this inference procedure to spiking data from synthetic and in vitro neuronal networks. The results show that the proposed procedure identifies the presence/absence of synaptic couplings fairly well including their signs, for the synthetic and experimental data. In particular, the results support that we can infer the physical connections of underlying systems in favorable situations, even when using the simple statistical model.