Modelling of surfactant-driven front instabilities in spreading bacterial colonies

TL;DR

This paper models how surfactant production and substrate wettability influence bacterial colony spreading and shape, explaining various growth patterns and instabilities through a hydrodynamic thin film approach.

Contribution

It introduces a hydrodynamic thin film model to analyze the impact of surfactant and wettability on bacterial colony morphology and growth patterns.

Findings

Reproduces four distinct colony growth types

Shows surfactant and wettability variations cause different instabilities

Explains finger formation and spreading arrest phenomena

Abstract

The spreading of bacterial colonies at solid-air interfaces is determined by the physico-chemical properties of the involved interfaces. The production of surfactant molecules by bacteria is a widespread strategy that allows the colony to efficiently expand over the substrate. On the one hand, surfactant molecules lower the surface tension of the colony, effectively increasing the wettability of the substrate, which facilitates spreading. On the other hand, gradients in the surface concentration of surfactant molecules result in Marangoni flows that drive spreading. These flows may cause an instability of the circular colony shape and the subsequent formation of fingers. In this work, we study the effect of bacterial surfactant production and substrate wettability on colony growth and shape within the framework of a hydrodynamic thin film model. We show that variations in the wettability and surfactant production are sufficient to reproduce four different types of colony growth, which have been described in the literature, namely, arrested and continuous spreading of circular colonies, slightly modulated front lines and the formation of pronounced fingers.