Particle Tracking and Extended Object Imaging by Interferometric Super Resolution Microscopy

TL;DR

This paper introduces an interferometric super-resolution microscopy technique that achieves sub-2 nm axial localization and real-time tracking of live biological samples through innovative phase retrieval and interference pattern analysis.

Contribution

It presents a novel interferometric microscope combined with a phase retrieval algorithm for super-resolution imaging and dynamic tracking of biological samples.

Findings

Achieved sub-2 nm axial resolution

Enabled real-time tracking at 5 Hz

Demonstrated imaging of live biological samples

Abstract

An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking utilizes the information stored in the interference pattern of both the illuminating incoherent light and the emitted light. By periodically shifting the interferometer phase and a phase retrieval algorithm we obtain information that allow localization with sub-2 nm axial resolution at 5 Hz.