Structural-elastic determination of the mechanical lifetime of biomolecules

TL;DR

This paper introduces a new analytical model based on structural-elastic properties to predict the force-dependent lifetime of biomolecules, overcoming limitations of previous energy landscape models.

Contribution

The model uniquely relates molecular structural-elastic features to force-dependent lifetimes without assuming a specific free energy landscape shape.

Findings

Successfully explains experimental force-lifetime data for various biomolecules.

Predicts structural-elastic properties of transition states.

Provides direct insights into molecular transition mechanisms.

Abstract

The lifetime of protein domains and ligand-receptor complexes under force is crucial for mechanosensitive functions, while many aspects of how force affects the lifetime still remain poorly understood. Here, we report a new analytical expression of the force-dependent molecular lifetime to understand transitions overcoming a single barrier. Unlike previous models derived in the framework of Kramers theory that requires a presumed one-dimensional free energy landscape, our model is derived based on the structural-elastic properties of molecules which is not restricted by the shape and dimensionality of the underlying free energy landscape. Importantly, the parameters of this model provide direct information of the structural-elastic features of the molecules between the transition and the native states. We demonstrate the applications of this model by applying it to explain complex force-dependent lifetime data for several molecules reported in recent experiments, and predict the structural-elastic properties of the transition states of these molecules.