Temporal scaling of the growth dependent radiative properties of microalgae

TL;DR

This study introduces the concept of temporal scaling functions to describe how microalgae's radiative properties change over growth time, enabling predictions of light interactions in photobioreactors.

Contribution

The paper presents experimental and theoretical evidence for a new temporal scaling model of microalgae radiative properties, aiding light field analysis in bioreactor design.

Findings

TSFs are approximately wavelength-independent across species.

Radiative properties at any growth stage can be predicted from stationary phase data.

Experimental validation confirms theoretical predictions of temporal scaling behavior.

Abstract

The radiative properties of microalgae are basic parameters for analyzing light field distribution in photobioreactors (PBRs). With the growth of microalgae cell, their radiative properties will vary with growth time due to accumulation of pigment and lipid, cell division and metabolism. In this work, we report both experimental and theoretical evidence of temporal scaling behavior of the growth dependent radiative properties of microalgae cell suspensions. A new concept, the temporal scaling function (TSF), defined as the ratio of absorption or scattering cross-sections at growth phase to that at stationary phase, is introduced to characterize the temporal scaling. The temporal evolution and temporal scaling characteristics of the absoroption and scattering cross-sections of three example microalgae species, Chlorella vulgaris, Chlorella pyrenoidosa, and Chlorella protothecoides, were experimentally studied at spectral range 380 to 850 nm. It is shown that the TSFs of the absorption and scattering cross-sections for different microalgae species are approximately constant at different wavelength, which confirms theoretical predictions very well. With the aid of the temporal scaling relation, the radiative properties of microalgae at any growth time can be calculated based on those measured at stationary phase. The findings of this work will help the understanding of time dependent radiative properties of microalgae and facilitate their applications in light field analysis in PBRs design.