Analysis of gamma-amino butyric acid in the germinated brown rice by pre-column derivatization with 2, 4-dinitrofluorobenzene

TL;DR

This paper develops a liquid chromatography method with pre-column derivatization for accurate GABA analysis in germinated brown rice, validating its precision and comparing it with amino acid analysis.

Contribution

The study introduces a validated LC method with derivatization for GABA in germinated brown rice, optimizing extraction and separation conditions.

Findings

GABA extraction time of 12 hours is optimal.

The method shows high linearity with a correlation coefficient of 0.999.

No significant difference compared to amino acid analyzer results.

Abstract

This paper reports the analytical method for gamma-amino butyric acid in the germinated brown rice by liquid chromatography. GABA was derivatized by pre-column derivatization with 2, 4-dinitrofluorobenzene. To separate GABA in the germinated brown rice extracts containing various amino acids, we reviewed the influence of the kind of separation columns and the gradient of mobile phase of ultra performance liquid chromatography. GABA was extracted with distilled water from the germinated brown rice with the optimum extraction time of 12 h. To validate this method the precision and the recovery were discussed. In the concentration range from 5 to 50 microgram per mililiter, the calibration curve for GABA was linear and the regression equation was obtained with correlation coefficient of 0.999. GABA was analyzed by amino acid analyzer in comparison with this method and the results of F-test and t-test indicated that there were no significant differences in accuracy and precision between two methods.