A generalised framework for detailed classification of swimming paths inside the Morris Water Maze
Avgoustinos Vouros, Tiago V. Gehring, Kinga Szydlowska, Artur Janusz,, Mike Croucher, Katarzyna Lukasiuk, Witold Konopka, Carmen Sandi, Zehai Tu,, Eleni Vasilaki

TL;DR
This paper introduces a robust, generalised framework and software for detailed classification of rodent swimming paths in the Morris Water Maze, enhancing behavioral analysis with minimal manual tuning.
Contribution
A new classification framework using majority voting that improves accuracy and usability for analyzing rodent trajectories in spatial learning tasks.
Findings
Framework boosts classification performance
Software provides user-friendly GUI for analysis
Method reduces need for manual parameter tuning
Abstract
The Morris Water Maze is commonly used in behavioural neuroscience for the study of spatial learning with rodents. Over the years, various methods of analysing rodent data collected in this task have been proposed. These methods span from classical performance measurements (e.g. escape latency, rodent speed, quadrant preference) to more sophisticated methods of categorisation which classify the animal swimming path into behavioural classes known as strategies. Classification techniques provide additional insight in relation to the actual animal behaviours but still only a limited amount of studies utilise them mainly because they highly depend on machine learning knowledge. We have previously demonstrated that the animals implement various strategies and by classifying whole trajectories can lead to the loss of important information. In this work, we developed a generalised and robust…
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