Software for full-color 3D reconstruction of the biological tissues internal structure

TL;DR

This paper introduces software that enables high-resolution, full-color 3D reconstruction of biological tissues from histological images, facilitating detailed internal structural analysis.

Contribution

The authors developed software that reconstructs full-color 3D images of biological tissues with high resolution from histological sections.

Findings

Achieved 3D reconstruction resolution of 5-10 μm.

Enabled arbitrary cross-section visualization.

Provided a method for detailed internal tissue analysis.

Abstract

A software for processing sets of full-color images of biological tissue histological sections is developed. We used histological sections obtained by the method of high-precision layer-by-layer grinding of frozen biological tissues. The software allows restoring the image of the tissue for an arbitrary cross-section of the tissue sample. Thus, our method is designed to create a full-color 3D reconstruction of the biological tissue structure. The resolution of 3D reconstruction is determined by the quality of the initial histological sections. The newly developed technology available to us provides a resolution of up to 5 - 10 {\mu}m in three dimensions.