Mechanical model of Golgi apparatus

TL;DR

This paper develops a mechanical model of the Golgi apparatus stack, explaining its shape, size, and number of cisternae through analytical and numerical methods, highlighting the role of adhesion and membrane properties.

Contribution

It introduces a minimal assumption mechanical model that predicts Golgi stack morphology and identifies key factors like adhesion strength influencing cisternae number.

Findings

Stack prefers a bent shape energetically

Adhesion strength determines cisternae number

Fenestrations affect overall stack structure

Abstract

The Golgi apparatus has intrigued researchers since its discovery and despite the advances, there are still many open questions in regards to its shape and function. Here, we propose a mechanical model of Golgi apparatus stack and explain its most elementary geometrical properties: the equilibrium number of cisternae, the stack size, and its general equilibrium shape. In order to gain comprehensive insight into the stack morphology, we combine both analytical and numerical methods. We successfully reconstruct the stack morphology using minimal assumptions, thus identifying the most dominant factors regulating the stack shape. We demonstrate that energy-wise the stack prefers an overall bent shape and show strong evidence that the adhesion strength determines the equilibrium number of cisternae per stack. We explore the morphological role of fenestrations and discuss their impact on the overall stack structure. We also comment on the effects of the membrane asymmetry on the shape of the cisternae and thus offer a broad steady-state study of the stack morphology and present a method that can be used also for other membrane-bound organelles.