Monovalent ions modulate the flux through multiple folding pathways of an RNA pseudoknot

TL;DR

This study combines experiments and simulations to reveal how monovalent ions influence the folding pathways of an RNA pseudoknot, showing that ionic strength modulates the flux between parallel folding routes and affects structural stability.

Contribution

It provides the first detailed analysis of how ionic conditions alter the folding pathways of an RNA pseudoknot using combined experimental and computational approaches.

Findings

Ionic strength modulates the flux between folding pathways.

Higher salt concentrations enable parallel folding pathways.

Folding order depends on hairpin stability and salt levels.

Abstract

The functions of RNA pseudoknots (PKs), which are minimal tertiary structural motifs and an integral part of several ribozymes and ribonucleoprotein complexes, are determined by their structure, stability and dynamics. Therefore, it is important to elucidate the general principles governing their thermodynamics/folding mechanisms. Here, we combine experiments and simulations to examine the folding/unfolding pathways of the VPK pseudoknot, a variant of the Mouse Mammary Tumor Virus (MMTV) PK involved in ribosomal frameshifting. Fluorescent nucleotide analogs (2-aminopurine and pyrrolocytidine) placed at different stem/loop positions in the PK, and laser temperature-jump approaches serve as local probes allowing us to monitor the order of assembly of VPK with two helices with different intrinsic stabilities. The experiments and molecular simulations show that at 50 mM KCl the dominant folding pathway populates only the more stable partially folded hairpin. As the salt concentration is increased a parallel folding pathway emerges, involving the less stable hairpin structure as an alternate intermediate. Notably, the flux between the pathways is modulated by the ionic strength. The findings support the principle that the order of PK structure formation is determined by the relative stabilities of the hairpins, which can be altered by sequence variations or salt concentrations. Our study not only unambiguously demonstrates that PK folds by parallel pathways, but also establishes that quantitative description of RNA self-assembly requires a synergistic combination of experiments and simulations.