Cross-Bridge Induced Adhesion of Red Blood Cells Assessed by Optical Tweezers

TL;DR

This study uses optical tweezers to investigate how red blood cells adhere in plasma and dextran solutions, confirming different mechanisms for each and providing insights into blood rheology.

Contribution

It demonstrates that RBC adhesion in dextran aligns with depletion layer models, while in plasma it follows a cross-bridge mechanism, confirmed by SEM imaging.

Findings

RBC adhesion in dextran matches depletion-induced models.

In plasma, RBC adhesion follows a cross-bridge mechanism.

SEM visualization confirms the cross-bridge adhesion in plasma.

Abstract

The reversible aggregation of red blood cells (RBCs) is a process of erythrocytes clumping that strongly influences the rheological properties of blood. The adhesion of RBCs has been studied extensively in the frame of cell-to-cell interaction induced by dextran macromolecules, whereas the data is lacking for native plasma solution. We apply optical tweezers to investigate the induced adhesion of RBCs in plasma and in dextran solution. Two hypotheses 'cross-bridges' and 'depletion layer' are being utilized to describe the mechanism of cells interaction, while both need to be confirmed experimentally. The results show that in dextran solution the interaction of adhering RBCs agrees well with the quantitative predictions obtained on the basis of a depletion-induced cells adhesion model, whereas a migrating 'cross-bridge' model is more appropriate for plasma. Furthermore, the 'cross-bridge' mechanism is confirmed by direct visualization of red blood cells adhesion utilizing scanning electron microscopy (SEM).