Differential Dynamic Microscopy microrheology of soft materials: a tracking-free determination of the frequency-dependent loss and storage moduli

TL;DR

This paper introduces DDM-$$r, a tracking-free microrheology method that accurately measures the frequency-dependent mechanical moduli of soft materials without calibration, overcoming limitations of traditional particle tracking techniques.

Contribution

The authors develop a novel, calibration-free DDM-$$r approach with a self-consistent analysis that extends microrheology to wider frequency ranges and challenging conditions.

Findings

Accurately determines mechanical moduli across a broad frequency spectrum.

Works in conditions where particle tracking microrheology fails.

Aligns with traditional rheology and DWS microrheology results.

Abstract

Particle tracking microrheology (PT-$\mu$r) exploits the thermal motion of embedded particles to probe the local mechanical properties of soft materials. Despite its appealing conceptual simplicity, PT-$\mu$r requires calibration procedures and operating assumptions that constitute a practical barrier to a wider adoption. Here we demonstrate Differential Dynamic Microscopy microrheology (DDM-$\mu$r), a tracking-free approach based on the multi-scale, temporal correlation study of the image intensity fluctuations that are observed in microscopy experiments as a consequence of the motion of the tracers. We show that the mechanical moduli of an arbitrary sample are determined correctly in a wide frequency range, provided that the standard DDM analysis is reinforced with a novel, iterative, self-consistent procedure that fully exploits the multi-scale information made available by DDM. Our approach to DDM-$\mu$r does not require any prior calibration, is in agreement with both traditional rheology and Diffusing Wave Spectroscopy microrheology, and works in conditions where PT-$\mu$r fails, providing thus an operationally simple, calibration-free probe of soft materials.