# Relation between Insertion Sequences and Genome Rearrangements in Pseudomonas aeruginosa

## TL;DR

This study investigates the relationship between insertion sequences, especially IS3, and genome rearrangements like inversions in Pseudomonas aeruginosa, using a new computational pipeline to analyze 18 complete genomes.

## Contribution

The paper introduces a novel pipeline for detecting IS distribution and links them to genome rearrangements, specifically identifying IS3's role in inversions.

## Key findings

- IS3 family is associated with genomic inversions
- New pipeline effectively detects IS distribution and rearrangements
- Rearrangements observed in 18 Pseudomonas aeruginosa genomes

## Abstract

During evolution of microorganisms genomes underwork have different changes in their lengths, gene orders, and gene contents. Investigating these structural rearrangements helps to understand how genomes have been modified over time. Some elements that play an important role in genome rearrangements are called insertion sequences (ISs), they are the simplest types of transposable elements (TEs) that widely spread within prokaryotic genomes. ISs can be defined as DNA segments that have the ability to move (cut and paste) themselves to another location within the same chromosome or not. Due to their ability to move around, they are often presented as responsible of some of these genomic recombination. Authors of this research work have regarded this claim, by checking if a relation between insertion sequences (ISs) and genome rearrangements can be found. To achieve this goal, a new pipeline that combines various tools have firstly been designed, for detecting the distribution of ORFs that belongs to each IS category. Secondly, links between these predicted ISs and observed rearrangements of two close genomes have been investigated, by seeing them with the naked eye, and by using computational approaches. The proposal has been tested on 18 complete bacterial genomes of Pseudomonas aeruginosa, leading to the conclusion that IS3 family of insertion sequences are related to genomic inversions.