Biobeam - Rigorous wave-optical simulations of light-sheet microscopy
Martin Weigert, Eugene W. Myers, Moritz Kreysing
TL;DR
Biobeam is an open-source software that simulates light-sheet microscopy using wave-optical methods, accurately modeling aberrations, artifacts, and distortions for improved understanding and design of microscopy systems.
Contribution
It introduces a GPU-accelerated, wave-optical simulation tool that faithfully reproduces complex optical phenomena in light-sheet microscopy.
Findings
Accurately models spatial aberrations and artifacts.
Enables computationally tractable simulations of image formation.
Supports design and analysis of light-sheet microscopy systems.
Abstract
Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables to simulate operational light-sheet microscopes by combining data from 10^5 - 10^6 multiplexed and GPU-accelerated point-spread-function calculations. The wave-optical nature of these simulations leads to the faithful reproduction of spatially varying aberrations, diffraction artifacts, geometric image distortions, adaptive optics, and emergent wave optical phenomena, and renders image-formation in light-sheet microscopy computationally tractable.
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