# Essentiality, conservation, evolutionary pressure and codon bias in bacterial genes

## TL;DR

This study reveals a universal exponential relationship between gene essentiality and conservation in bacteria, identifying core essential genes with conserved functions and codon bias, relevant for synthetic biology and antimicrobial targets.

## Contribution

It demonstrates a universal exponential relation between gene essentiality and conservation across bacteria and characterizes two distinct gene groups based on conservation and codon bias.

## Key findings

- Essential genes are highly conserved and under strong purifying selection.
- Two gene groups identified: core essential and less conserved nonessential genes.
- Core essential genes are potential targets for antimicrobial drugs.

## Abstract

Essential genes constitute the core of genes which cannot be mutated too much nor lost along the evolutionary history of a species. Natural selection is expected to be stricter on essential genes and on conserved (highly shared) genes, than on genes that are either nonessential or peculiar to a single or a few species. In order to further assess this expectation, we study here how essentiality of a gene is connected with its degree of conservation among several unrelated bacterial species, each one characterised by its own codon usage bias. Confirming previous results on E. coli, we show the existence of a universal exponential relation between gene essentiality and conservation in bacteria. Moreover we show that, within each bacterial genome, there are at least two groups of functionally distinct genes, characterised by different levels of conservation and codon bias: i) a core of essential genes, mainly related to cellular information processing; ii) a set of less conserved nonessential genes with prevalent functions related to metabolism. In particular, the genes in the first group are more retained among species, are subject to a stronger purifying conservative selection and display a more limited repertoire of synonymous codons. The core of essential genes is close to the minimal bacterial genome, which is in the focus of recent studies in synthetic biology, though we confirm that orthologs of genes that are essential in one species are not necessarily essential in other species. We also list a set of highly shared genes which, reasonably, could constitute a reservoir of targets for new anti-microbial drugs.