# Exhaled breath barbotage: a new method for pulmonary surfactant dysfunction assessment

## TL;DR

This paper introduces a novel non-invasive method called exhaled breath barbotage (EBB) for collecting pulmonary surfactant from exhaled aerosols, enabling the study of surfactant surface properties to assess lung dysfunction.

## Contribution

The study presents a new sampling technique (EBB) and a data analysis algorithm to evaluate pulmonary surfactant properties from exhaled aerosols, facilitating non-invasive lung diagnostics.

## Key findings

- Differentiated surfactant surface properties between healthy and diseased subjects.
- Demonstrated the method's potential for pulmonary surfactant dysfunction assessment.
- Compared surfactant isotherms with artificial surfactant samples.

## Abstract

Exhaled air contains aerosol of submicron droplets of the alveolar lining fluid (ALF), which are generated in the small airways of a human lung. Since the exhaled particles are micro-samples of the ALF, their trapping opens up an opportunity to collect non-invasively a native material from respiratory tract. Recent studies of the particle characteristics (such as size distribution, concentration and composition) in healthy and diseased subjects performed under various conditions have demonstrated a high potential of the analysis of exhaled aerosol droplets for identifying and monitoring pathological processes in the ALF. In this paper we present a new method for sampling of aerosol particles during the exhaled breath barbotage (EBB) through liquid. The barbotage procedure results in accumulation of the pulmonary surfactant, being the main component of ALF, on the liquid surface, which makes possible the study its surface properties. We also propose a data processing algorithm to evaluate the surface pressure ($\pi$) -- surface concentration ($\Gamma$) isotherm from the raw data measured in a Langmuir trough. Finally, we analyze the $(\pi-\Gamma)$ isotherms obtained for the samples collected in the groups of healthy volunteers and patients with pulmonary tuberculosis and compare them with the isotherm measured for the artificial pulmonary surfactant.