Violation of the sphericity assumption and its effect on Type-I error rates in repeated measures ANOVA and multi-level linear models (MLM)

TL;DR

This study investigates how violations of the sphericity assumption affect Type I error rates in repeated measures ANOVA and multi-level linear models, considering up to nine measurement occasions through extensive simulation.

Contribution

It provides new insights into the impact of sphericity violations on error rates across different models and sample sizes, especially with many measurement occasions.

Findings

MLM-UN shows significant bias with small samples and many measurement occasions.

Greenhouse-Geisser correction is slightly conservative under certain conditions.

Huynh-Feldt correction is recommended when sphericity is violated with small samples.

Abstract

This study aims to investigate the effects of violations of the sphericity assumption on Type I error rates for different methodical approaches of repeated measures analysis using a simulation approach. In contrast to previous simulation studies on this topic, up to nine measurement occasions were considered. Therefore, two populations representing the conditions of a violation vs. a non-violation of the sphericity assumption without any between-group effect or within-subject effect were created and 5,000 random samples of each population were drawn. Finally, the mean Type I error rates for Multilevel linear models (MLM) with an unstructured covariance matrix (MLM-UN), MLM with compound-symmetry (MLM-CS) and for repeated measures analysis of variance (rANOVA) models (without correction, with Greenhouse-Geisser-correction, and Huynh-Feldt-correction) were computed. To examine the effect of both the sample size and the number of measurement occasions, sample sizes of n = 20, 40, 60, 80, and 100 were considered as well as measurement occasions of m = 3, 6 and 9. For MLM-UN, the results illustrate a massive progressive bias for small sample sizes (n =20) and m = 6 or more measurement occasions. This effect could not be found in previous simulation studies with a smaller number of measurement occasions. The mean Type I error rates for rANOVA with Greenhouse-Geisser-correction demonstrate a small conservative bias if sphericity was not violated, sample sizes were small (n = 20), and m = 6 or more measurement occasions were conducted. The results plead for a use of rANOVA with Huynh-Feldt-correction, especially when the sphericity assumption is violated, the sample size is rather small and the number of measurement occasions is large. MLM-UN may be used when the sphericity assumption is violated and when sample sizes are large.