# Nanoscale membrane budding induced by CTxB on quasi-one component lipid bilayers detected by polarized localization microscopy

## TL;DR

This study uses polarized localization microscopy to reveal that cholera toxin subunit B (CTxB) can induce nanoscale membrane budding in supported lipid bilayers, with curvature-dependent CTxB clustering and diffusion changes, shedding light on membrane reorganization mechanisms.

## Contribution

The paper demonstrates that CTxB inherently induces membrane bending at the nanoscale, providing new insights into its role in membrane dynamics independent of other cellular factors.

## Key findings

- Membrane buds with <50 nm radius detected, growing to >200 nm.
- CTxB is >10x more concentrated on positive curvature and >25x on negative curvature.
- Slowed diffusion of lipids and CTxB at membrane buds indicating increased viscosity.

## Abstract

For endocytosis and exocytosis, membranes transition between planar, budding, and vesicular topographies through nanoscale reorganization of lipids, proteins, and carbohydrates. However, prior attempts to understand the initial stages of nanoscale bending have been limited by experimental resolution. Through the implementation of polarized localization microscopy (PLM), this manuscript reports the inherent membrane bending capability of cholera toxin subunit B (CTxB) in quasi-one component supported lipid bilayers. Membrane buds were first detected with <50 nm radius, grew to >200 nm radius, and extended into longer tubules with dependence on the membrane tension and CTxB concentration. Compared to the concentration of the planar supported lipid bilayers, CTxB was >10x more concentrated on the positive curvature top and >25x more concentrated on the negative Gaussian curvature neck of the nanoscale membrane buds. This finding elucidates prior observations by correlating CTxB clustering and diffusion to CTxB-induced membrane bending. CTxB is frequently used as a marker for liquid-ordered lipid phases; however, the coupling between CTxB and membrane bending provides an alternate understanding of CTxB-induced membrane reorganization. Single-particle tracking was performed on both lipids and CTxB to reveal the correlation between single-molecule diffusion, CTxB accumulation, and membrane topography. Slowed lipid and CTxB diffusion was observed at the nanoscale buds locations, suggesting a local increase in membrane viscosity or molecular crowding upon membrane bending. These results suggest inherent CTxB-induced membrane bending as a mechanism for initiating CTxB internalization in cells that is initially independent of clathrin, caveolin, actin, and lipid phase separation.

---
Source: https://tomesphere.com/paper/1703.08865