Mathematical Modelling and Analysis of the Brassinosteroid and Gibberellin Signalling Pathways and their Interactions

TL;DR

This paper develops and analyzes mathematical models for brassinosteroid and gibberellin hormone signalling pathways in plants, highlighting the dominant role of transcription factor interactions in pathway crosstalk and stability.

Contribution

It introduces new coupled PDE-ODE models for BR and GA pathways, incorporating spatial heterogeneity and crosstalk mechanisms, with parameter estimation based on experimental data.

Findings

BZR-DELLA interaction significantly influences pathway dynamics.

Perturbations in GA pathway have larger effects than those in BR.

Crosstalk perturbations greatly impact BR pathway dynamics.

Abstract

The plant hormones brassinosteroid (BR) and gibberellin (GA) have important roles in a wide range of processes involved in plant growth and development. In this paper we derive and analyse new mathematical models for the BR signalling pathway and for the crosstalk between the BR and GA signalling pathways. To analyse the effects of spatial heterogeneity of the signalling processes, along with spatially-homogeneous ODE models we derive coupled PDE-ODE systems modelling the temporal and spatial dynamics of molecules involved in the signalling pathways. The values of the parameters in the model for the BR signalling pathway are determined using experimental data on the gene expression of BR biosynthetic enzymes. The stability of steady state solutions of our mathematical model, shown for a wide range of parameters, can be related to the BR homeostasis. Our results for the crosstalk model suggest that the interaction between transcription factors BZR and DELLA exerts more influence on the dynamics of the signalling pathways than BZR-mediated biosynthesis of GA, suggesting that the interaction between transcription factors may constitute the principal mechanism of the crosstalk between the BR and GA signalling pathways. In general, perturbations in the GA signalling pathway have larger effects on the dynamics of components of the BR signalling pathway than perturbations in the BR signalling pathway on the dynamics of the GA pathway. The perturbation in the crosstalk mechanism also has a larger effect on the dynamics of the BR pathway than of the GA pathway. Large changes in the dynamics of the GA signalling processes can be observed only in the case where there are disturbances in both the BR signalling pathway and the crosstalk mechanism.