Cellulyzer - Automated analysis and interactive visualization/simulation of select cellular processes

TL;DR

Cellulyzer is a comprehensive software suite integrated into ImageJ that automates tracking, analysis, visualization, and simulation of cellular processes, facilitating optimized bio-image analysis and experimental validation.

Contribution

It introduces a set of integrated, interactive tools for automated cellular image analysis, visualization, and simulation, compatible across multiple platforms and accessible via web browsers.

Findings

Automated tracking and analysis of cellular images.

Interactive visualization schemes for cellular processes.

Simulation tools for validating and optimizing image acquisition.

Abstract

Here we report on a set of programs developed at the ZMBH Bio-Imaging Facility for tracking real-life images of cellular processes. These programs perform 1) automated tracking; 2) quantitative and comparative track analyses of different images in different groups; 3) different interactive visualization schemes; and 4) interactive realistic simulation of different cellular processes for validation and optimal problem-specific adjustment of image acquisition parameters (tradeoff between speed, resolution, and quality with feedback from the very final results). The collection of programs is primarily developed for the common bio-image analysis software ImageJ (as a single Java Plugin). Some programs are also available in other languages (C++ and Javascript) and may be run simply with a web-browser; even on a low-end Tablet or Smartphone. The programs are available at https://github.com/nurlicht/CellulyzerDemo