Non-contact luminescence lifetime cryothermometry for macromolecular crystallography

TL;DR

This paper introduces a non-contact luminescence lifetime cryothermometry technique for precise, remote temperature monitoring of protein crystals during macromolecular crystallography, enhancing thermal characterization at cryogenic temperatures.

Contribution

The work presents a novel luminescence lifetime-based thermometry system integrated into MX beamline setups, with calibration and validation over a wide cryogenic temperature range.

Findings

Temperature measurement uncertainty of ±1.6 K in 30-150 K range

Sample mount thickness significantly affects temperature distribution

The system accurately tracks sample holder temperature during experiments

Abstract

A novel technique for remote, non-contact, in situ monitoring of the protein crystal temperature has been developed for the new I23 beamline at the Diamond Light Source, a facility dedicated to macromolecular crystallography (MX) with long-wavelength X-rays. The temperature is derived from the temperature-dependant decay time constant of luminescence from a minuscule scintillation sensor (0.05 mm3) located in very close proximity to the sample under test. In this work we present the underlying principle of cryogenic luminescence lifetime thermometry, discuss the features of the detection method, the choice of temperature sensor and demonstrate how the temperature monitoring system was integrated within the viewing system of the end-station used for the visualisation of protein crystals. The thermometry system was characterised using a Bi4Ge3O12 (BGO) crystal scintillator that exhibits good responsivity of the decay time constant as function of temperature over a wide range (8-270 K). The scintillation sensor was calibrated and the uncertainty of the temperature measurements over the primary operation temperature range of the beamline (30-150 K) was assessed to be +-1.6 K. It has been shown that the temperature of the sample holder, measured using the luminescence sensor, agrees well with the expected value. The technique was applied to characterise the thermal performance of different sample mounts that have been used in MX experiments at the I23 beamline. The thickness of the mount is shown to have the greatest impact upon the temperature distribution across the sample mount. Altogether these tests and findings demonstrate the usefulness of the thermometry system in highlighting the challenges that remain to be addressed for the in-vacuum MX experiment to become a reliable and indispensable tool for structural biology