# Origin of spatial organization of DNA-polymer in bacterial chromosomes

## TL;DR

This study demonstrates that a small percentage of specific cross-links in a ring polymer model can significantly influence the spatial organization of bacterial DNA, with predictions validated through simulations.

## Contribution

It introduces a novel approach showing how specific cross-links derived from contact maps shape DNA organization in bacterial chromosomes.

## Key findings

- Specific cross-links lead to distinct DNA structures
- Random cross-links produce different organization
- Monte Carlo simulations validate predictions

## Abstract

In-vivo DNA organization at large length scales ($\sim 100nm$) is highly debated and polymer models have proved useful to understand the principle of DNA-organization. Here, we show that $<2$% cross-links at specific points in a ring polymer can lead to a distinct spatial organization of the polymer. The specific pairs of cross-linked monomers were extracted from contact maps of bacterial DNA. We are able to predict the structure of 2 DNAs using Monte Carlo simulations of the bead-spring polymer with cross-links at these special positions. Simulations with cross-links at random positions along the chain show that the organization of the polymer is different in nature from the previous case.