Time-multiplexed structured illumination using a DMD for optical diffraction tomography

TL;DR

This paper introduces a new illumination control method for optical diffraction tomography using a DMD, which improves image quality by eliminating unwanted diffraction artifacts and enables detailed 3D imaging of biological samples.

Contribution

The paper presents a novel time-multiplexed structured illumination technique with a DMD that reduces diffraction artifacts in ODT, enhancing image quality and quantitative analysis.

Findings

High RI sensitivity of .15 b1 10-4 achieved

Successful 3D RI reconstructions of biological samples

Elimination of unwanted diffracted beams improves image quality

Abstract

We present a novel illumination control technique for optical diffraction tomography (ODT). Various spatial frequencies of beam illumination were controlled by displaying time-averaged sinusoidal patterns using a digital micromirror device (DMD). Compared to the previous method using binary Lee holograms, the present method eliminates unwanted diffracted beams which may deteriorate the image quality of the ODT. We demonstrated the capability of the present method by reconstructing three-dimensional refractive index (RI) distributions of various samples, with high RI sensitivity (\sigma_\Delta n = 3.15 +/- 10-4), and reconstructing 3-D RI tomograms of biological samples, which provided quantitative biochemical and morphological information about the samples.