Calibrating photon counts from a single image
Rainer Heintzmann, Peter K. Relich, Robert P.J. Nieuwenhuizen, Keith, A. Lidke, Bernd Rieger

TL;DR
This paper introduces a simple, retroactive method for calibrating detector gain directly from a single image, eliminating the need for multiple calibration images and maintaining comparable precision to traditional methods.
Contribution
The proposed method allows gain calibration from a single image by quantifying out-of-band information, simplifying the process and enabling retroactive application.
Findings
Comparable precision to traditional calibration methods
Applicable to both simulated and experimental data
Eliminates need for multiple calibration images
Abstract
Current methods for detector gain calibration require acquisition of tens of special calibration images. Here we propose a method that obtains the gain from the actual image for which the photon count is desired by quantifying out-of-band information. We show on simulation and experimental data that our much simpler procedure, which can be retroactively applied to any image, is comparable in precision to traditional gain calibration procedures.
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Taxonomy
TopicsAdvanced Fluorescence Microscopy Techniques · CCD and CMOS Imaging Sensors · Advanced Optical Sensing Technologies
