Axial Super-resolution Evanescent Wave Tomography

TL;DR

This paper introduces AxSET, a novel method that achieves axial super-resolution in 3D optical tomography using evanescent waves, enabling detailed imaging beyond traditional limits without extra hardware.

Contribution

The paper presents a Fourier reconstruction-based technique for axial super-resolution tomography using evanescent waves, validated through simulations and experiments on biological samples.

Findings

Achieved ~130 nm axial resolution in microtubule imaging.

Demonstrated compatibility with existing TIRF microscopes.

No additional optical components needed.

Abstract

Optical tomographic reconstruction of a 3D nanoscale specimen is hindered by the axial diffraction limit, which is 2-3 times worse than the focal plane resolution. We propose and experimentally demonstrate an axial super-resolution evanescent wave tomography (AxSET) method that enables the use of regular evanescent wave microscopes like Total Internal Reflection Fluorescence Microscope (TIRF) beyond surface imaging, and achieve tomographic reconstruction with axial super-resolution. Our proposed method based on Fourier reconstruction achieves axial super-resolution by extracting information from multiple sets of three-dimensional fluorescence images when the sample is illuminated by an evanescent wave. We propose a procedure to extract super-resolution features from the incremental penetration of an evanescent wave and support our theory by 1D (along the optical axis) and 3D simulations. We validate our claims by experimentally demonstrating tomographic reconstruction of microtubules in HeLa cells with an axial resolution of $\sim$130 nm. Our method does not require any additional optical components or sample preparation. The proposed method can be combined with focal plane super-resolution techniques like STORM and can also be adapted for THz and microwave near-field tomography.