In silico modeling of the rheological properties of covalently crosslinked collagen triple helices

TL;DR

This study introduces a mathematical model to predict the rheological properties of covalently crosslinked collagen hydrogels, aiding the rational design of biomimetic materials with controlled properties.

Contribution

The paper presents a novel in silico model treating collagen triple helices as semi-flexible polymers, accurately predicting experimental rheological trends.

Findings

Model successfully predicts storage modulus based on collagen concentration.

Model captures effects of different crosslinking segments on rheology.

Experimental data aligns with computational predictions.

Abstract

Biomimetic hydrogels based on natural polymers are a promising class of biomaterial, mimicking the natural extra-cellular matrix of biological tissues and providing cues for cell attachment, proliferation and differentiation. With a view to providing an upstream method to guide subsequent experimental design, the aim of this study was to introduce a mathematical model that described the rheological properties of a hydrogel system based on covalently crosslinked collagen triple helices. In light of their organization, such gels exhibit limited collagen bundling that cannot be described by existing fibril network models. The model presented here treats collagen triple helices as discrete semi-flexible polymers, permits full access to metrics for network microstructure, and should provide a comprehensive understanding of the parameter space associated with the development of such multi-functional materials. Triple helical hydrogel networks were experimentally obtained via reaction of type I collagen with both aromatic and aliphatic diacids. The complex modulus G* was found from rheological testing in linear shear and quantitatively compared to model predictions. In silico data from the computational model successfully described the experimental trends in hydrogel storage modulus with either (i) the concentration of collagen triple helices during crosslinking reaction or (ii) the type of crosslinking segment introduced in resulting hydrogel networks. This approach may pave the way to a step change in the rational design of biomimetic triple helical collagen systems with controlled multi-functionality.