Effect of Nanodiamond surfaces on Drug Delivery Systems

TL;DR

This study investigates how nanodiamond surfaces influence tRNA dynamics, revealing that the presence of nanodiamonds accelerates tRNA motion within a specific temperature range, which has implications for drug delivery systems.

Contribution

It provides detailed analysis of tRNA behavior on nanodiamond surfaces using neutron scattering and molecular dynamics, highlighting the impact on RNA flexibility and dynamics.

Findings

tRNA shows increased mobility on ND surfaces between 220K and 310K

tRNA dynamics follow Mode Coupling theory's logarithmic decay

Presence of ND causes swelling and faster motion of tRNA

Abstract

The prospect of RNA nanotechnology is increasing because of its numerous potential applications especially in medical science. The spherical Nanodiamonds (NDs) are becoming popular because of their lesser toxicity, desirable mechanical, optical properties, functionality and available surface areas. On other hand RNAs are stable, flexible and easy to bind to the NDs. In this work, we have studied the tRNA dynamics on ND surface by high-resolution quasi-elastic neutron scattering spectroscopy and all atom molecular dynamics simulation technique to understand how the tRNA motion is affected by the presence of ND. The flexibly of the tRNA is analyzed by the Mean square displacement analysis that shows tRNA have a sharp increase around 230K in its hydrated form. The intermediate scattering function (ISF) representing the tRNA dynamics follows the logarithmic decay as proposed by the Mode Coupling theory (MCT). But most importantly the tRNA dynamics is found to be faster in presence of ND within 220K to 310K compared to the freestanding ones. This is as we have shown, is because of the swollen RNA molecule due the introduction of hydrophilic ND surface.