Creation of a tight PSF array for scanning structured illumination via phase retrieval

TL;DR

This paper introduces a novel multi-spot PSF design for a two-photon scanning structured illumination microscope, utilizing phase retrieval to enhance imaging speed and robustness in biological samples.

Contribution

A new multi-spot PSF generated via phase retrieval algorithms for improved two-photon structured illumination microscopy.

Findings

Simulated effective multi-spot PSF for TPE-SIM

Demonstrated viability of the proposed PSF design

Proposed a simulation-based multi-spot PSF scanning SIM microscope

Abstract

In this work, we propose a structured illumination (SI) method based on a two-photon excitation (TPE) scanning laser beam. Advantages of TPE methods include optical sectioning, low photo-toxicity, and robustness in the face of sample induced scattering. We designed a novel multi-spot point spread function (PSF) for a fast, two-photon scanning SIM microscope. Our multi-spot PSF is generated with a phase retrieval algorithm. We show how to obtain the phase distribution and then simulate the effect of this distribution on a spatial light modulator (SLM), which produces the multi-spot PSF in the object plane of the microscope. We produce simulations that show the viability of this method. The results are simulated and a multi-spot PSF scanning SIM microscope is proposed.