Laparoscopic real-time en-face differential optical topography of near-surface heterogeneity by using an applicator probe with the maximum-density radially alternating illumination and detection fiber-channels

TL;DR

This paper introduces a laparoscopic applicator probe with dense radially alternating fibers for real-time en-face optical mapping of near-surface heterogeneity, aiding intraoperative margin assessment in minimally invasive cancer surgeries.

Contribution

The development of a compact, high-density fiber-optic probe capable of real-time, en-face topographic mapping of tissue heterogeneity during laparoscopic procedures is novel.

Findings

Real-time mapping of near-surface heterogeneity achieved at 1.25Hz.

Probe fits in a 12mm port with 128 fibers for illumination and detection.

Effective visualization of subsurface margin up to 3mm deep.

Abstract

We demonstrate a laparoscopic applicator probe and a method thereof for real-time en-face topographic mapping of near-surface heterogeneity for potential use in intraoperative margin assessment during minimally invasive oncological procedures. The probe fits in a 12mm port and houses at its maximum 128 copper-coated 750um fibers that form radially alternating illumination (70 fibers) and detection (58 fibers) channels. By simultaneously illuminating the 70 source channels of the probe that is in contact with a scattering medium and concurrently measuring the light diffusely propagated to the 58 detector channels, the presence of near-surface optical heterogeneities can be resolved in an en-face 9.5mm field-of-view in real-time. Visualization of a subsurface margin of strong attenuation contrast at a depth up to 3mm is demonstrated at one wavelength at a frame rate of 1.25Hz.