LC/ESI-MS analysis of underivatized amino acids and mass spectrum
Yoshinori Takano, Yoshito Chikaraishi, Naohiko Ohkouchi
TL;DR
This paper presents a method for analyzing underivatized amino acids using LC/ESI-MS, providing mass spectra and fragmentation patterns to support identification and quantification, with optimized chromatography for isotope studies.
Contribution
It introduces an LC/ESI-MS technique for amino acids without derivatization, including optimized chromatography and diagnostic ions for improved analysis.
Findings
Mass spectra and fragmentation patterns identified.
Optimized chromatography with NFPA.
Supports qualitative and quantitative amino acid analysis.
Abstract
We report the method of LC/ESI-MS analysis of underivatized amino acids with corresponding mass spectrum and fragmentation patterns. Diagnostic product ions determined by mass spectrometry can support the qualitative identification and quantitative estimation of individual amino acids. Therefore, the optimization of chromatographic separation using the ion-pairing reagent (i.e., Nonafluoropentanoic acid, NFPA) is useful for the evaluation of target amino acid and for further compound-specific nitrogen isotope studies of amino acids.
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Taxonomy
TopicsIsotope Analysis in Ecology · Mass Spectrometry Techniques and Applications · Metabolomics and Mass Spectrometry Studies