A MoS2-based capacitive displacement sensor for DNA sequencing

TL;DR

This paper introduces a novel capacitive displacement sensor based on MoS2 nanoribbons for DNA sequencing, achieving high accuracy and throughput without requiring nanopores, offering a promising cost-effective sequencing technology.

Contribution

It presents the first MoS2-based capacitive sensor for DNA sequencing, combining base-specific detection with high speed and simplified design without nanopores.

Findings

Achieves 79-86% raw detection accuracy at ~70 million bases/sec

Demonstrates reliable detection of repeated DNA motifs

Proposes a simplified sensor geometry without nanopores

Abstract

We propose an aqueous functionalized molybdenum disulfide nanoribbon suspended over a solid electrode as the first capacitive displacement sensor aimed at determining the DNA sequence. The detectable sequencing events arise from the combination of Watson-Crick base-pairing, one of nature's most basic lock-and-key-binding mechanisms, with the ability of appropriately sized atomically thin membranes to flex substantially in response to sub-nanonewton forces. We employ carefully designed numerical simulations and theoretical estimates to demonstrate excellent (79 % to 86 %) raw target detection accuracy at ~70 million bases per second and electrical measurability of the detected events. In addition, we demonstrate reliable detection of repeated DNA motifs. Finally, we argue that the use of a nanoscale opening (nanopore) is not requisite for the operation of the proposed sensor and present a simplified sensor geometry without the nanopore as part of the sensing element. Our results therefore potentially suggest a realistic, inherently base-specific, high-throughput electronic DNA sequencing device as a cost-effective de-novo alternative to the existing methods.