Intracellular regulatory control of gene expression process

TL;DR

This paper introduces intracellular feedback control strategies using synthetic genetic circuits to regulate gene expression dynamics, demonstrating their effectiveness through numerical simulations.

Contribution

It proposes novel genetic comparator circuits for intracellular regulation, including Genetic Relay Switch, Shifted Subtractor, and Bistable Comparator, for precise gene expression control.

Findings

Effective regulation of gene expression demonstrated in simulations

Design of novel genetic comparator circuits

Potential for precise intracellular gene control

Abstract

This paper presents an intracellular feedback control strategy, to regulate the gene expression process dynamics. For this purpose, two types of genetic circuits are designed in order to compare concentrations of the input transcription factor (the desired input) and the protein produced by the expression of target gene (process output). Genetic Relay Switch and Shifted Subtractor are proposed and employed to design Discrete Comparator. Also, another type of genetic comparator is presented named Bistable Comparator. These synthetic comparators are used to produce the required amount of the protein that activates the expression of the target gene. Numerical simulation results, demonstrate the effectiveness of the designed controllers.