Selective Trapping of DNA using Glass Microcapillaries

TL;DR

This paper demonstrates a cost-effective method for trapping and delivering DNA using glass microcapillaries, combining experimental work with simulation to optimize parameters for analyte capture.

Contribution

It introduces a novel integrated experimental and simulation approach for selective DNA trapping in microcapillaries, enabling tunable separation based on electrophoretic mobility.

Findings

Microcapillaries can efficiently trap DNA using electrokinetic flows.

Simulation accurately predicts trapping efficiency across parameters.

Method enables low-cost analyte separation for lab-on-a-chip devices.

Abstract

We show experimentally that a cheap glass microcapillary can accumulate {\lambda}-phage DNA at its tip and deliver the DNA into the capillary using a combination of electro-osmotic flow, pressure-driven flow, and electrophoresis. We develop an efficient simulation model for this phenomenon based on the electrokinetic equations and the finite-element method. Using our model, we explore the large parameter space of the trapping mechanism by varying the salt concentration, the capillary surface charge, the applied voltage, the pressure difference, and the mobility of the analyte molecules. Our simulation results show that this system can be tuned to capture a wide range of analyte molecules, such as DNA or proteins, based on their electrophoretic mobility. Our method for separation and pre-concentration of analytes has implications for the development of low-cost lab-on-a-chip devices.