Stimulated low-frequency Raman scattering in tobacco mosaic virus suspension

TL;DR

This study investigates stimulated low-frequency Raman scattering in tobacco mosaic virus suspensions, revealing a new Raman line and measuring key parameters like frequency shift, efficiency, and threshold for the first time.

Contribution

It reports the first observation and measurement of stimulated low-frequency Raman scattering in TMV suspensions, including the frequency shift, conversion efficiency, and threshold.

Findings

SLFRS observed at 2 cm-1 shift in TMV in buffer

Conversion efficiency and threshold measured for the first time

Stimulated Raman scattering depends on the virus environment

Abstract

Laser pulses interaction with tobacco mosaic virus (TMV) in Tris-HCl pH7.5 buffer and in water has been investigated. 20 ns ruby laser pulses have been used for excitation. Spectrum of the light passing through the sample was registered with the help of Fabri-Perot interferometer. In the case of TMV in water we observed in the spectrum only one line of the exciting laser light, for TMV in Tris-HCl pH7.5 buffer second line appeared, corresponding to the stimulated low-frequency Raman scattering (SLFRS) on the breathing radial mode of TMV. SLFRS frequency shift by 2 cm-1, (60 GHz), conversion efficiency and threshold are measured for the first time to the best of our knowledge.